Measurement of anti proliferative activity experiment

The image of the slides were checked and captured by an Axiophot-2 Universal microscope Zeiss connected with a computer for documentation. All the animals were observed and weighed daily. Briefly, the assay buffer and substrate were equilibrated to room temperature, and the buffer was transferred to and gently mixed with the substrate to obtain a homogeneous solution.

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The anti-cancer potential of Melicope ptelefolia

The anti-proliferative activities of sinigrin were studied in a model of carcinogen-induced hepatotoxicity in rats. Wells with serum free medium were used as negative control.

At the end of the experiment, all rats were killed by carbon dioxide asphyxiation and the blood was collected. The suspension was transferred into a microcentrifuge tube. The susceptibility to EGCG treatment is largely dependent on cell type.

Food and water were given ad libitum. The gene expression of sinigrin-treated HepG2 cells revealed that sinigrin induced apoptosis via a pdependent pathway. The cell suspension solution was centrifuged at 1, rpm for 3 minutes. Glucosinolates have been reported to exhibit different pharmacological properties in vitro [1] — [4].

However, the precise details of the pharmacological activity of sinigrin in rats are not currently available. Contributed to the writing of the manuscript: Slide-checking was necessary to assure the best staining effect.

The details of primer mixes used for each gene are as follows: However, the precise details of the pharmacological activity of sinigrin in rats are not currently available.

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Abstract Background The chemopreventive effect of green tea polyphenols, such as - -epigallocatechingallate EGCGhas been well demonstrated in cell culture studies. This article has been cited by other articles in PMC.

Many methods have been developed to measure cell proliferation including those based on direct counting of viable cells, measurement of metabolic activity and cellular DNA content. Statistics One-way ANOVA was performed to determine the statistical significance of differences among treated and untreated groups.

Animal Treatment Sprague Dawley SD rats were induced with carcinogens in the in vivo experiments according to the previously established method [11]. Rats were orally administered with sinigrin on a daily basis for three months before sacrifice. The protocol of the experiment was the same as for the AST assay.

GST-p Immuno-staining Slides with liver sections were placed on a slide holder, dewaxed and rehydrated with xylene and different concentrations of ethanol as mentioned previously. An Axiophot-2 Universal microscope Zeiss connected with computer were used to capture cell contents images using Spot 32 image capture software Diagnostic Instruments.

All the animals were observed and weighed daily.

Anti-Proliferative Activities of Sinigrin on Carcinogen-Induced Hepatotoxicity in Rats

Measurement of mitochondrial metabolic rate using MTT 3- 4,5-Dimethylthiazolyl -2,5-diphenyltetrazolium bromide and MTS 3- 4,5-dimethylthiazolyl 3-carboxymethoxyphenyl 4-sulfophenyl -2H-tetrazolium to indirectly reflect viable cell numbers has been widely applied [7] - [9][11].

The results suggest the liver function of the positive control group untreated rats having liver cancers was attenuated compared with the treatment group. Slides were then immersed in hematoxylin for about 3 minutes and allowed to develop the coloration for several minutes in water, followed by brief destaining with acidic ethanol and water.

Sinigrin was found to significantly inhibit the proliferation of liver tumor cells; the number of surface tumors in the rat liver was dramatically reduced. Readings were then taken at 30 second intervals for a period of 3 minutes. Better treatments for liver cancer are thus clearly needed.

All the experiments were repeated three times. The results showed that after doxorubicin treatment the number of surface tumors in the rat liver was not affected whereas the group of rats that was treated with sinigrin displayed considerably reduced numbers of surface tumors Figure 5.

Vascular endothelial growth factor induces proliferation of breast cancer cells and inhibits the anti-proliferative activity of anti-hormones Yayun Liang1, Rolf A Brekken2 and Salman M Hyder1,3 1Dalton Cardiovascular Research Center, University of Missouri, Research Park Drive, Columbia, MissouriUSA 2Hamon Center for Therapeutic.

We investigated the effect of the LXR agonist, T, on ovarian cancer cell proliferation and apoptosis as a potential therapeutic agent. T treatment resulted in a significant (P.

Apr 16,  · The anti-proliferative activity of EGCG has been demonstrated in various cancer cell lines, however, the half maximal inhibitory concentration (IC 50) values from different studies vary greatly from tens to hundreds of µmol/L of EGCG.

The susceptibility to EGCG treatment is. Various natural compounds were reported to persuade cell cytotoxicity through targeting and downregulation of the HIF The genus Salvia is a rich source of bioactive terpenoids which show promising anti-cancer activities.

Here, the identification of natural anti-proliferative compound targeting the HIF-1α expression was reported. The results from the anti-proliferative activity assays are shown in Table 1, Table 2.

All the compounds can be divided into three groups, namely 4, 5 and 6, according to their structural scaffolds.

When tested against the SK-N-MC and HCT cell lines, compounds 4a and 4b had limited anti-proliferative activity.

Anti-Proliferative Activities of Sinigrin on Carcinogen-Induced Hepatotoxicity in Rats

lying anti-proliferative activity of T-bet remain to be eluci- A scintillation beta counter was used to measure radioactivity in DNA recovered from the cells (Microbeta TopCount, Perkin Elmer, Shelton, CT, USA). experiments were performed using 6 mice for each experiment.

Measurement of anti proliferative activity experiment
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